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. 2015 Apr 29;10(1):31–36. doi: 10.3892/etm.2015.2457

Figure 4.

Figure 4.

Quantitative polymerase chain reaction (qPCR) analysis. Expression of the Sca-1 gene was determined by qPCR. Adherent and nonadherent cultured cells between passages 9 and 11 were used in the experiment. The data are the average of three independent experiments. (A) Sca-1 expression in the nonadherent cultured cells at 24 h, as compared with the adherent cultured cells at 24 h (P>0.05). (B) Sca-1 expression in the nonadherent cultured cells at 72 h compared with the adherent cultured cells at 72 h (**P<0.01). (C) Sca-1 expression in the nonadherent cultured cells at 5 days, as compared with the adherent cultured cells at 5 days (*P<0.05). These experiments demonstrated that the mRNA expression levels of Sca-1 in the mesenchymal stem cells were downregulated after 72 h in the nonadherent culture; however, Sca-1 mRNA expression recovered following culture for 5 days in the adherent culture conditions. Sca, stem cell antigen; Ad, adherent; Nonad, nonadherent.