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. 2015 Apr 15;17(4):428–440. doi: 10.1007/s10126-015-9632-1

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© The Author(s) 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Two-dimensional TLC analysis of ¹⁴C-LMC produced by the coccolithophorid E. huxleyi NIES 837 under continuous illumination for 24 h. a Fractionation protocol for the preparation of LMC for TLC analysis. b Autoradiogram. c Mannitol spot (shown by arrow) visualized by KMnO₄ reagent. d A merged image of the autoradiogram (b) and chemical staining of mannitol (c). The TLC images presented here are representative of two independent experiments using LP cells. e Quantification of ¹⁴C-mannitol (expressed as % of total ¹⁴C fixation). Results of two independent experiments (exp. 1 and 2) are shown