Fig. 5.

Changes in ¹⁴C in various fractions during light/dark transition in the coccolithophore E. huxleyi NIES837. First, cells were photosynthetically labeled with ¹⁴C using NaH¹⁴CO₃ as the substrate for 10 h, and then transferred to dark conditions. a Changes in ¹⁴C incorporation into LP cells (closed squares) and SP cells (open squares) expressed as actual radioactivity (dpm cell⁻¹). b Relative amount of ¹⁴C under dark conditions (% change) in LP (closed squares) and SP cells (open squares), respectively. c, d Changes in ¹⁴C incorporation into each metabolite in the LMC-dominant fraction (crosses), APext (closed circles), lipids other than alkenones (open diamonds), alkenones (closed diamonds), APint (open circles), and β-glucan (closed triangles) expressed as actual activity (dpm cell⁻¹) in LP and SP cells, respectively. e, f Graphs with magnified y-axis of Fig. 5c, d for lipids other than alkenones (open diamonds), alkenones (closed diamonds), APint (open circles), and β-glucan (closed triangles) in LP and SP cells, respectively. Representative results of two independent experiments are shown