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. 2015 Jul 1;5:11347. doi: 10.1038/srep11347

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Copyright © 2015, Macmillan Publishers Limited

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(a) Bio-layer interferometry kinetic binding data for the interaction between probe-immobilized thrombin and RNA pools from indicated selections rounds. Toggle-25 and scrToggle-25 were included as positive and negative controls, respectively. (b) Comparison of fluorescently labeled 5–12, Toggle-25, scrToggle-25 and capture oligonucleotide binding to tRBCs versus sRBCs. (c) A conserved motif present in Round 3 and 5 selected pools is indicated, and its location within a stem-loop element within the secondary structure predicted for aptamer 5–12 is indicated. (d) Evaluation of the importance of this conserved motif to 5–12 thrombin binding affinity was assessed by measuring thrombin binding affinity of: (i) 5–12mini, in which the 5–12 was truncated while preserving the motif; and (ii) 5–12mut, in which the motif was mutated in the context of full-length aptamer.