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. 2015 Jul 1;5:11629. doi: 10.1038/srep11629

Figure 4. HLT-iASPP Pro interacts with iASPP Ank-SH3 in vitro.

Figure 4

Nickel affinity pulldown of iASPP Ank-SH3 by HLT-iASPP Pro, SDS PAGE gel coomassie staining results. Nickel-NTA beads were incubated with HLT-iASPP Pro or with buffer or HLT fusion domain alone, for 1 h at 4 °C. The samples were centrifuged and then incubated with iASPP Ank-SH3 for 2 h at 4 °C. The samples were centrifuged again and the beads were washed 3 times. The beads were eluted by boiling them in SDS solution. Ank-SH3 eluted from the beads only in the presence of HLT-iASPP Pro.