Figure 1. Principle of the method:

a suspension of F. prausnitzii in non-growing conditions metabolizes glucose and reduces RF to RFH₂. RFH₂ concentration is continuously monitored by chronoamperometry on a glassy carbon RDE, providing in real time the metabolic reaction rate. Some metabolic kinetic parameters such as k_cat for RF or glucose consumption can be obtained once coupled with cell count (flow cytometry).