Table 1. Comparison of electrochemical vs. spectrophotometric detection of RF reduction.
RDE | 96 well plate reader | |
---|---|---|
Minimal [F. prausnitzii] (cells.mL−1) | 8.6 × 104 | 2.6 × 106 |
Minimal initial [RF] required (μM) | 0.28 | 4.8 |
Minimal RF consumption rate (M.min−1) | 5.3 × 10−9 | 7.6 × 10−6 |
Minimal recording time (min) | 0.5–2 | >5 |
Maximal linear RF removal (min) | 500 | 1 − 132* |
*determined for n = 3 wells 4.8–170 μM RF and 2.6 × 106– 2.8 × 109cells.mL−1.