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. 2015 Jul 1;5:11617. doi: 10.1038/srep11617

Table 1. Production of and 13C abundance in P(3HB) on [1-13C1]-glucose, and carbon yield of P(3HB) on glucose with R. eutropha H16G and CBB cycle-inactivated strains.

R. eutropha strain Time (h) P(3HB)a (g/L) 13C abundance in P(3HB)b (%) P(3HB) yieldc (g/g-glucose)
H16G 2 0.21 ± 0.21 2.09 ± 0.00 0.345
12 1.61 ± 0.06 5.64 ± 0.00
H16G∆cbbR 2 0.20 ± 0.00 1.79 ± 0.00 0.294
12 1.38 ± 0.03 2.34 ± 0.00
H16G∆∆cbbLS 2 0.22 ± 0.04 1.68 ± 0.08 0.286
12 1.00 ± 0.02 1.91 ± 0.02

aP(3HB) produced from [1-13C1]-glucose by 2-step cultivation.

bMeans of 13C/12C ratio calculated from isotopomer abundances of the two fragments (m/z 45 and 87) derived from 3HB methyl ester.

cDetermined from relationship between P(3HB) production and glucose consumption obtained by 6 ~ 8 independent 2-step cultivations for various incubation periods.