Table 1. Production of and 13C abundance in P(3HB) on [1-13C1]-glucose, and carbon yield of P(3HB) on glucose with R. eutropha H16G and CBB cycle-inactivated strains.
R. eutropha strain | Time (h) | P(3HB)a (g/L) | 13C abundance in P(3HB)b (%) | P(3HB) yieldc (g/g-glucose) |
---|---|---|---|---|
H16G | 2 | 0.21 ± 0.21 | 2.09 ± 0.00 | 0.345 |
12 | 1.61 ± 0.06 | 5.64 ± 0.00 | ||
H16G∆cbbR | 2 | 0.20 ± 0.00 | 1.79 ± 0.00 | 0.294 |
12 | 1.38 ± 0.03 | 2.34 ± 0.00 | ||
H16G∆∆cbbLS | 2 | 0.22 ± 0.04 | 1.68 ± 0.08 | 0.286 |
12 | 1.00 ± 0.02 | 1.91 ± 0.02 |
aP(3HB) produced from [1-13C1]-glucose by 2-step cultivation.
bMeans of 13C/12C ratio calculated from isotopomer abundances of the two fragments (m/z 45 and 87) derived from 3HB methyl ester.
cDetermined from relationship between P(3HB) production and glucose consumption obtained by 6 ~ 8 independent 2-step cultivations for various incubation periods.