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. 2015 Jul 1;5:11599. doi: 10.1038/srep11599

Figure 1. Inhibitory effects of sulfated polysaccharides on C. parvum sporozoite invasion of HCT-8 cells.

Figure 1

C. parvum sporozoites were inoculated to HCT-8 cells in RPMI-1640 medium containing each sulfated polysaccharide. The number of parasites left in the HCT-8 cells was counted per 100 fields of view by use of fluorescence microscopy. Each assay was performed in independent triplicates, and means ± standard deviations are shown. (a) Inhibitory efficacy of sulfated polysaccharides. Each sulfated polysaccharide was added at the concentration of 1, 10, or 100 μg /mL in RPMI-1640 medium. (b) Inhibitory efficacy of heparin tested over a wide range of concentrations. Heparin was added at the concentrations of ten-fold serial dilutions from 2,000,000 to 2 ng/mL in RPMI-1640 medium.