Figure 5. Heparin-binding property of rCpEF1α.

To examine whether recombinant CpEF1α binds to heparin, a pull down assay was conducted. Purified GST-CpEF1α and GST-CpActin were incubated with heparin agarose beads (Input; lanes 1 and 4), and beads were then pulled down. Heparin-unbound phase and heparin-binding proteins are shown for GST-CpEF1α (Lanes 2 and 3), and for GST-CpActin (Lanes 5 and 6), respectively. Arrows indicate GST-fused recombinant proteins.