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. 2015 Jul 1;5:11599. doi: 10.1038/srep11599

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Copyright © 2015, Macmillan Publishers Limited

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(a) The purified recombinant proteins were analyzed by SDS-PAGE and immunoblotting. The recombinant proteins were expressed in E. coli and purified by GST affinity chromatography. The purified proteins were concentrated by ultra-filtration. The black and white arrows indicate rCpEF1α and rGST, respectively. (b) Flow cytometry of HCT-8 cells incubated with recombinant protein. HCT-8 cells were incubated with each recombinant protein, reacted with rabbit α-GST antibody IgG, stained with Alexa 488-conjugated α-rabbit IgG goat antibody, and then subjected to flow cytometry analysis. The cells were gated on forward/side-light scatter to distinguish them from debris. The cells incubated with rCpEF1α (dark gray-filled histogram) showed higher fluorescence intensity than those incubated with rGST (bright gray-filled).