Table 1. Heparin-binding proteins of C. parvum identified by using nano-LC/MS/MS.

Sample	Protein	MW(Da)	Scorea	Peptideb	Coveragec(%)
1	hypothetical protein with a signal peptide	111,402	741	29	35
	conserved hypothetical protein	123,651	442	26	31
	domain KOG1015, transcription regulator XNP/ATRX, DEAD-box superfamily, signal peptide	91,962	429	25	32
	signal peptide, large secreted protein	120,615	327	24	24
	hypothetical protein containing a signal peptide	114,961	136	7	8
	signal peptide, large protein	147,338	50	5	4
	phosphoenolpyruvate carboxylase	130,753	34	1	0
2	acetaldehyde reductase plus alcohol dehydrogenase (AdhE) of possible bacterial origin	94,675	509	25	38
	Eft2p GTpase; translation elongation factor 2 (EF-2)	93,215	501	26	38
	unconventional myosin	91,421	390	22	25
	zincin/aminopeptidase N like metalloprotease	105,843	382	21	25
	heat shock protein 90 (Hsp90), signal peptide plus ER retention motif	89,137	336	19	31
	glycogen phosphorylase	104,146	301	23	27
	KH domain protein	91,196	220	9	11
	Hsp90	82,302	186	13	24
	conserved hypothetical protein	102,374	102	3	4
	CDC48 like AAA ATPase ortholog	90,504	43	2	2
	conserved hypothetical protein	123,651	35	2	1
3	Actin	42,147	401	23	61
	elongation factor 1 alpha	48,131	379	20	62
	phosphoglycerate kinase 1	43,286	274	13	39
	60S ribosomal protein-like, putative	43,224	224	13	44
	hypothetical protein	34,486	198	11	28
	hypothetical protein, signal peptide, predicted secreted protein	40,831	178	8	30
	conserved protein of possible plant or bacterial origin	47,123	65	4	13
	elongation factor EF1-gamma (glutathione S-transferase family)	43,061	47	3	7
	protein kinase, cAMP-dependent, catalytic chain	45,424	44	2	4
	ATP-dependent RNA helicase, putative	46,008	36	2	5
	protein disulfide isomerase, signal peptide, ER retention motif	50,484	35	3	6
	membrane associated thioredoxin	42,072	34	2	6
	26S proteasome regulatory subunit S10b like AAA + ATpase	45,515	33	4	7

Heparin-binding proteins identified throughnano-LC/MS/MS analysis are listed. Proteins whose ion score were >31 are shown.

^aProtein score. This number reflects the combined scores of all observed mass spectra that can bematched to amino acid sequenceswithin that protein. A higher score indicates a more confident match.

^bThe number of peptides included in each identified protein.

^cThe ratio of the sequence of the identified protein occupied by the found peptides.