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. 2015 Jul 1;5:10270. doi: 10.1038/srep10270

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Copyright © 2015, Macmillan Publishers Limited

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COS-7 cells were transfected with the best cyan, yellow and red FP linked trios. a. Here showing: Tq2-Cherry-YPet. The two acceptors were selectively and sequentially photobleached. Cherry was photobleached by collimated 561 nm laser illumination (4500 ms pulse at 95 W/cm²; 15 cycles), followed by YPet photobleaching by collimated 515 nm laser illumination (1500 ms pulse at 72 W/cm²; 15 cycles). b. The average intensity for ROIs during the acquisition shows the expected trends as the yellow and cyan donors are dequenched. c. Characteristic FRET efficiencies for various Cyan-Cherry-YPet constructs, all error bars are standard deviation with N = 30.