Figure 6.

Inhibitory effect of rosmarinic acid and arbutin on receptor activator of nuclear factor-κB ligand (RANKL)-induced resorption pit area. Murine macrophage/osteoclast precursor cell line RAW 264.7 cells were seeded in a 24-well plate at a density of 1×10⁴ cells/well and treated with RANKL and (A) rosmarinic acid or (B) arbutin at the indicated concentration. After 11 days culture, the cells were was hed with phosphate-buffered saline and removed from the well walls with 6% sodium hypochlorite solution. Images of the resorption pit area were captured. Data are representative of results from three separate experiments (n=3).