Figure 1.

Downregulation of CHOP protein levels by IL-6. The mRNA and protein expression levels of CHOP were determined by quantitative polymerase chain reaction and western blot analysis, respectively, in (A and B) MC3T3-E1 cells and (C and D) primary mouse BMCs treated with PBS or IL-6 (10 ng/ml) for 24 and 48 h. CHOP, CCAAT-enhancer-binding homologous protein; STAT3, signal transducer and activator of transcription 3; P-STAT3, phosphorylated-STAT3; PBS, phosphate-buffered saline; IL-6, interleukin-6; BMC, bone marrow cell.