Figure 2.

STAT3 overexpression reduces CHOP protein content in osteoblasts. The mRNA and protein expression levels of CHOP were determined by quantitative polymerase chain reaction and western blot analysis, respectively, in (A and B) MC3T3-E1 cells and (C and D) primary mouse BMCs transfected with lentiviruses containing an EV or CA-STAT3 for 24 and 48 h. CHOP, CCAAT-enhancer-binding homologous protein; EV, empty vector; CA-STAT3, constitutively active-signal transducer and activator of transcription 3; BMC, bone marrow cell.