Figure 4.

Quantitative polymerase chain reaction analyses of the expression of the dental pulp cell (DPC)-characteristic genes in dental pulp and gingival tissues. Tissues (Pulp-1, Pulp-2, Pulp-3, Pulp-4 and Gingiva-1) were obtained from several donors (Table I). The pulp cells DPCs-1 were cultured with Dulbecco's modified Eagle's medium in the presence of 10% fetal bovine serum. Of note, DPCs-1, Pulp-1 and Gingiva-1 were obtained from the same donor. The values for tissues are averages of duplicate determinations, and those for DPCs are mean ± standard deviation of four cultures. MSX1, msh homeobox 1; MSX2, msh homeobox 2; TBX2, T-box 2; ENTPD1, ectonucleoside triphosphate diphosphohydrolase 1.