Figure 2.

Direct sequencing results for the polymorphisms of the LMP2 and LMP7 genes. The polymorphisms were detected by restriction fragment length polymorphism assay and confirmed by direct polymerase chain reaction sequencing. The single base indicated by a black arrowhead is the site of the small nucleotide polymorphism. (A) Representative DNA sequencing results of the (a) Arg/Arg, (b) Arg/His and (c) His/His genotypes of the Arg to His mutation at codon 60 of the LMP2 gene. (B) Representative DNA sequencing results of the (a) Gln/Gln, (b) Gln/Lys and (c) Lys/Lys genotypes of the Gln to Lys mutation at codon 145 of the LMP7 gene. LMP, low molecular weight protein.