Figure 3.

H19 regulates proliferation and epithelial-to-mesenchymal transition. The cells were treated with (A) pCDNA or pCDNA-H19 and (B) siRNA/control or siRNA/H19. (C) E-cadherin, Vimentin and fibronectin protein expression levels in Eca-109 cells transfected with pCDNA, pCDNA-H19, siRNA/control and siRNA/H19 were analyzed by western blotting. GAPDH was used as a loading control. The average values of the integrated optical densities were determined by analyzing five fields per slide. The data are presented as the mean ± standard error of the mean. *P<0.05. siRNA, small interfering RNA.