Figure 5.

Subcellular localization of claudin-3 in mock versus siRNA-transfected MCF-7 and MDA-MB-415 by differential detergent fractionation of cells. Subcellular fractions of mock or siRNA-transfected (A) MCF-7 and (B) MDA-MB-415 cells were immunoblotted with anti-claudin-3. The majority of claudin-3 was localized in the membrane fraction of non-transfected cells. Lower levels of claudin-3 were also observed in the cytosolic, nuclear and cytoskeletal fractions. siRNA-mediated suppression of claudin-3 resulted in an overall decrease in the level of claudin-3 protein in all sub-cellular fractions, with the greatest claudin-3 signal in the membrane fraction. Cyt, cytosolic; Mem, membranous; Nuc, nuclear; Csk, cytoskeletal; Cldn, claudin.