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. 2015 Jun 15;4:e07295. doi: 10.7554/eLife.07295

Figure 5. WRKY33 controls ABA-mediated plant susceptibility to B. cinerea 2100.

(A) Growth phenotypes of WT, wrky33, nced3 nced5, and wrky33 nced3 nced5 Arabidopsis plants at 4 weeks under short day conditions. (B) B. cinerea infection phenotypes 3 days post inoculation of WT, wrky33, nced3, nced5, nced3 nced5, and wrky33 nced3 nced5. (C) B. cinerea biomass quantification on indicated Arabidopsis genotypes. For fungal biomass determination, the relative abundance of B. cinerea and Arabidopsis DNA was determined by qPCR employing specific primers for BcCutinase A and AtSKII, respectively. (D) Exogenous application of ABA (10 μM) directly to infection droplets on wrky33 nced3 nced5 leaves partially rendered plants susceptible to B. cinerea 2100. Upon completion of the infection experiments (3 dpi), leaves were detached and photographed. For the infections, one or two 2 μl droplets containing 2.5 × 105 spores were applied to each leaf.

DOI: http://dx.doi.org/10.7554/eLife.07295.012

Figure 5.

Figure 5—figure supplement 1. B. cinerea 2100 infection phenotypes of mature 4-week old leaves derived from aba2-12 and aba3-1 mutant plants.

Figure 5—figure supplement 1.

Pictures were taken 3 days post inoculation of two 2 μl droplets containing 2.5 × 105 spores to each leaf (leaves were detached after completion of the experiment for photographic purposes only).
DOI: http://dx.doi.org/10.7554/eLife.07295.013
Figure 5—figure supplement 2. B. cinerea 2100 infection phenotypes of mature 4-week old leaves derived from wrky33 nced3 and wrky33 nced5 mutant plants.

Figure 5—figure supplement 2.

Pictures were taken 3 days post inoculation of two 2 μl droplets containing 2.5 × 105 spores to each leaf (leaves were detached after completion of the experiment for photographic purposes only).
DOI: http://dx.doi.org/10.7554/eLife.07295.014
Figure 5—figure supplement 3. Phenotype of WT Col-0 plants treated with ABA.

Figure 5—figure supplement 3.

Infection droplets with ABA (10 μM; red arrows) or without ABA (black arrows) were applied to 4-week old leaves. No alteration of the WT resistant phenotype was observed. Picture was taken 3 days post inoculation of two 2 μl droplets containing 2.5 × 105 spores to each leaf.
DOI: http://dx.doi.org/10.7554/eLife.07295.015
Figure 5—figure supplement 4. B. cinerea 2100 infection phenotypes of mature 4-week old leaves derived from cyp707a1, cyp707a2 and cyp707a3 mutant plants.

Figure 5—figure supplement 4.

Pictures were taken 3 days post inoculation of two 2 μl droplets containing 2.5 × 105 spores to each leaf (leaves were detached after completion of the experiment for photographic purposes only).
DOI: http://dx.doi.org/10.7554/eLife.07295.016