Figure 2.

Hispolon inhibited TPA-induced migration and invasion of MDA-MB-231 cells. (A) MDA-MB-231 cells were treated with hispolon for 2 h followed by TPA (160 nM) treatment for 24 h. The anti-invasive properties of hispolon were detected by Matrigel-based Transwell invasion assay. (B) Cells were treated as described previously and the antimigration ability was detected by wound-healing assay. (C) Relative quantification of the cell invasion and migration. All the data are presented as the mean ± standard deviation of three independent experiments. *P<0.05 and ^#P<0.05, vs. TPA alone-treated group; **P<0.05 and ^##P<0.05 vs. DMSO control. TPA, 12-O-tetradecanoylphorbol-13-acetate; DMSO, dimethyl sulfoxide; TPA+H-10, 160 nM TPA combined with 10 µM hispolon; TPA+H-20, 160 nM TPA combined with 20 µM hispolon; TPA+H-40, 160 nM TPA combined with 40 µM hispolon.