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Suppression of PDH E1α reduces abundance of metabolites downstream of PDH. a Western blot analysis for PDH E1α. Cyclophilin B was used as a loading control. b PDH activity from control cells and cells expressing PDHA1 shRNAs. c, d Intracellular levels of acetyl-carnitine and citrate. Values are the average and SD of biological triplicates, except (b) which represents technical triplicates. *P < 0.05; **P < 0.005
