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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Hypertension. 2015 Apr 27;65(6):1288–1297. doi: 10.1161/HYPERTENSIONAHA.115.05290

Figure 2.

Figure 2

A, Map of adenoviral vector construct containing a noradrenergic neuron-specific promoter, (PRSx8), NOS1-AP (neuronal nitric oxide synthase adaptor protein [CAPON]) gene, and red mCherry fluorescent protein (Ad.PRSx8-mCherry/CAPON). As a control, the same construct without CAPON gene insert was used (Ad.PRSx8-mCherry). B, Coimmunostaining of stellate neurons with anti–tyrosine hydroxylase (TH, green) and mCherry (red) tagged viral construct showed viral transduction in sympathetic neurons. Nuclear staining with DAPI (4′,6-diamidino-2-phenylindole) is in blue. Scale bar, 25 μm. C, Representative Western blot and group mean data showing CAPON expression (74 kDa) and nNOS expression in stellate ganglia from 4-week-old SHR 3 days after transduction with Ad.PRSx8-mCherry/CAPON (Ad.CAPON, n=6) and Ad.PRSx8- mCherry (empty, n=6; **P<0.01, unpaired t test).