Table 1. Primers used for PPAD single-point mutagenesis.
| Name | Sequence (5′−>3′) | Name | Sequence (5′−>3′) |
|---|---|---|---|
| pTCowPPADf | tgcagctagctccttaaggtggatggatatacg | H236Fs | ggcaagtatttggcaccgaa |
| pTCowPPADr | ctgacgcatgccaatcggtcgttagagttctcc | H236Rs | gatatattcgccgttcggatct |
| W127AFt | tcttacgctacacgcgactataccggttggttcgcaa | H236AFt | aacgctgtggactgttggggcaagtatttggcaccgaa |
| W127ARt | gtcgcgtgtagcgtaagagtcagttttcgcaatgatgaa | H236ARt | ccaacagtccacagcgttgatatattcgccgttcggatct |
| D130Fs | tataccggttggttcgcaa | H236NFt | aacaatgtggactgttggggcaagtatttggcaccgaa |
| D130Rs | gtcagttttcgcaatgatgaa | H236NRt | ccaacagtccacattgttgatatattcgccgttcggatct |
| D130AFt | tcttactggacacgcgcttataccggttggttcgcaa | D238AFt | aaccatgtggcttgttggggcaagtatttggcaccgaa |
| D130ARt | agcgcgtgtccagtaagagtcagttttcgcaatgatgaa | D238ARt | ccaacaagccacatggttgatatattcgccgttcggatct |
| D130NFt | tcttactggacacgcaactataccggttggttcgcaa | D238NFt | aaccatgtgaattgttggggcaagtatttggcaccgaa |
| D130NRt | gttgcgtgtccagtaagagtcagttttcgcaatgatgaa | D238NRt | ccaacaattcacatggttgatatattcgccgttcggatct |
| R152AFs | cctcgtcctaacgatgatga | D239AFt | aaccatgtggacgcttggggcaagtatttggcaccgaa |
| R152ARs | cacgagacctactttgttcgtatc | D239ARt | ccaagcgtccacatggttgatatattcgccgttcggatct |
| R152AFt | gactttatttataacgcccctcgtcctaacgatgatga | D239Eft | aaccatgtggacgaatggggcaagtatttggcaccgaa |
| R152ARt | ggcgttataaataaagtccacgagacctactttgttcgtatc | D239ERt | ccattcgtccacatggttgatatattcgccgttcggatct |
| R154Fs | gaattccccaaatacgaagc | D239SFt | aaccatgtggactcttggggcaagtatttggcaccgaa |
| R154Rs | gcggttataaataaagtccacg | D239SRt | ccaagagtccacatggttgatatattcgccgttcggatct |
| R154AFt | cctgctcctaacgatgatgaattccccaaatacgaagc | N297AFs | acaacagggtatttgttcctg |
| R154ARt | atcatcgttaggagcagggcggttataaataaagtccacg | N297ARs | tgtacggttgttcattggtg |
| R154EFt | cctgaacctaacgatgatgaattccccaaatacgaagc | N297AFt | cggcttctctgattctgaacaacagggtatttgttcctg |
| R154ERt | atcatcgttaggttcagggcggttataaataaagtccacg | N297ARt | tcagaatcagagaagccgtgtacggttgttcattggtg |
| T180Fs | acatgacggacggatatgga | C351Fs | ggtagcggataagggctatctc |
| T180Rs | tgagcttcatcccgaacatc | C351Rs | agggcatctgttcctaaccaag |
| T180AFt | agcaggctggtggcaactacatgacggacggatatgga | C351AFt | gcatgctcgtactcacgaggtagcggataagggctatctc |
| T180ARt | agttgccaccagcctgcttgagcttcatcccgaacatc | C351ARt | tcgtgagtacgagcatgcagggcatctgttcctaaccaag |
| G182Aft | agcagaccggtgctaactacatgacggacggatatgga | C351SFt | gcattctcgtactcacgaggtagcggataagggctatctc |
| G182ARt | agttagcaccggtctgcttgagcttcatcccgaacatc | C351SRt | tcgtgagtacgagaatgcagggcatctgttcctaaccaag |
Restriction enzyme recognition sequences are underlined.