Characterization of BNC–LP complexes.
Notes: (A) Transmission electron microscopy (TEM) photographs of (a) the BNC–LP complexes prepared at room temperature under neutral conditions, (b) the interface between BNCs and LP in the boxed area of (a), White arrowheads indicate the interface between BNCs and LP. (c) The BNC–LP complexes prepared at 70°C under acid conditions (namely virosomes), and (d) LP. (B) The virosomes treated with trypsin were separated by SDS-PAGE, and then immunoblotted with anti-preS1 antibody (upper panel) and anti-S antibody (lower panel). Asterisks indicate the bands of anti-preS1 immunoreactive proteins (~48 and ~43 kDa) and anti-S immunoreactive proteins (~16, ~19, ~26, and ~29 kDa). The sizes (C) and ζ-potentials (D) of virosomes (green), virosomes containing DOX (red), and LPs (blue) were measured by DLS. Scale bars represent 100 nm.
Abbreviations: BNC, bionanocapsule; LP, liposome; Try, trypsin; Ab, antibody; SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis; DOX, doxorubicin; DLS, dynamic light scattering.