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. 2015 Jun 1;17(3):170–180. doi: 10.1089/cell.2014.0085

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Copyright 2015, Mary Ann Liebert, Inc.

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FIG. 3. — (A) Outgrowth morphology observed after applying methods 2, 3, and 4. (A and B) Blastocyst outgrowth at day 5 (A), and day 3 post trypsinization of the outgrowth (B) in a mixture of primed hESCs and naïve N2B27 medium. (C and D) Day 5 after plating a day 6 blastocyst in N2B27+2i+LIF (C), and day 3 after trypsinization of the outgrowth (D). (E and F) Day 5 after plating two day 5 blastocysts in N2B27+2i+LIF. D5, day 5; D3, day 3. (B) Outgrowth morphology observed after applying methods 6, 7, and 8. Notice the progenitor-like cells in the squares. (A and B) Day 5 after plating two day 6 blastocysts in presence of Wnt5A. (C and D) Day 3 outgrowth in presence of epinephrine after plating a whole day 5 blastocyst (C) or an isolated ICM (D). (E and F) Day 3 outgrowth in presence of Wnt5A and epinephrine after plating a whole day 5 blastocyst (E) or an isolated ICM (F). D5, day 5; D3, day 3.