FIG. 1.

Prediction and analysis of Nanog methylation status. (A) Twelve CpG sites (seven in the promoter, one in the 5′-UTR, and four in the first exon, respectively) were analyzed in the Nanog sequence around the transcription start site (TSS) by the MethPrimer program. TSS was designated as +1. (B) Nanog methylation status in sperm (B1), oocytes (B2), and donor cells (B3). (Black or white circles) Methylated or unmethylated CpG sites; (gray circles) mutated and/or single-nucleotide polymorphism (SNP) variation at certain CpG sites. (C) Average methylation levels of the Nanog promoter, 5′-UTR, and the first exon in sperm, oocytes, and donor cells.