Transcript levels of Nanog, Oct4, and Sox2 in IVF, NT-CON, NT-AZA, and NT-TSA embryos. (A) Relative transcript levels of Nanog in IVF, NT-CON, NT-AZA, and NT-TSA embryos. (A′) Relative transcript levels of Nanog at the one-cell, two-cell, four-cell, eight-cell, and blastocyst stages of IVF, NT-CON, NT-AZA, and NT-TSA embryos. (B) Relative transcript levels of Oct4 at the one-cell, two-cell, four-cell, and blastocyst stages of IVF, NT-CON, NT-AZA, and NT-TSA embryos. (C) Relative transcript levels of Sox2 at the one-cell, two-cell, four-cell, and blastocyst stages of IVF, NT-CON, NT-AZA, and NT-TSA embryos. The transcript abundance for each gene in oocytes at the MII stage was considered as the control. The data are expressed as mean±SEM. a–dValues of a given gene in a certain group (A) or at a certain stage (A′, B, and C) in columns with different superscripts differ significantly (p<0.05).