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Effects of constitutive Nanog expression on the calcification of C3H10T1/2 cells. (A) von Kossa staining of GFP- or Nanog-expressing cells cultured in the presence or absence of rhBMP-2 at 21 days. (B) High-magnification image of von Kossa staining of GFP- or Nanog-expressing cells cultured in the presence of rhBMP-2 at 21 days. Bar, 200 μm.
