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. 2015 Jun 1;17(3):227–234. doi: 10.1089/cell.2014.0059

FIG. 2.

FIG. 2.

Runx1 as a downstream effector of Nanog. Results of the real-time RT-PCR conducted to measure the expression levels of Runx1 in GFP-expressing cells and Nanog-expressing cells cultured with or without rhBMP-2 for the indicated times (group A) (A) and those cultured with or without rhBMP-2 for 3 days (group B) (B). Quantities of mRNA are indicated as the levels in 1 μg of total RNA. Bars, means±standard deviation (SD) for three wells/group. (*) Significant stimulation at p<0.001 compared to GFP. (#) Significant stimulation at p<0.005 compared to GFP. We used multiple combinations of cells that had the same origin (parental cells) in each experiment. We measured the expression levels of Runx1, Col1, and osteocalcin in Nanog-expressing cells transfected with control siRNA or Runx1 siRNA and cultured with rhBMP-2 (C) or without rhBMP-2 (D) by quantitative real-time RT-PCR. Quantities of mRNA are indicated as the levels in 1 μg of total RNA. Bars, means±standard deviation (SD) for three wells/group. (*) Significant inhibition at p<0.01 compared to the control siRNA. (#) Significant inhibition at p<0.05 compared to the control siRNA. d, days.