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. 2015 Jun 1;17(3):227–234. doi: 10.1089/cell.2014.0059

FIG. 3.

FIG. 3.

Runx3 as a downstream effector of Nanog. We measured the expression levels of Runx3 in GFP-expressing cells and Nanog-expressing cells cultured with or without rhBMP-2 for the indicated times (group A) (A) and those cultured with or without rhBMP-2 for 3 days (group B) (B) by quantitative real-time RT-PCR. Quantities of mRNA are indicated as the levels in 1 μg of total RNA. Bars, means±standard deviation (SD) for three wells/group. (*) Significant stimulation at p<0.001 compared to GFP. (#) Significant stimulation at p<0.05 compared to GFP. We used multiple combinations of cells as described in the Fig. 2 legend. (C) We measured the expression levels of Runx3, Col1, and osteocalcin in Nanog-expressing cells transfected with control siRNA or Runx3 siRNA and cultured with rhBMP-2 by quantitative real-time RT-PCR. Quantities of mRNA are indicated as the levels in 1 μg of total RNA. Bars, means±standard deviation (SD) for three wells/group. (*) Significant inhibition at p<0.01 compared to the control siRNA. d, days.