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Wild type but not mutant NGLY1 restores the ddVenus fluorescence. (A) Western blot analysis of hNGLY1 overexpression by electroporation in fibroblasts. Protein Coomassie blue stain serves as loading control. (B) Patient fibroblasts were transfected with ddVenus together with the same amount of plasmids containing human wild-type NGLY1 (hNGLY1) or human NGLY1 mutant C309A. This figure is available in black and white in print and in color at Glycobiology online.
