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. 2014 Dec 26;2:33–38. doi: 10.1016/j.mex.2014.12.003

Fig. 1.

Fig. 1

Schematic overview of a closed method for frequent 24 h blood sample collection. (A) Continuous infusion. (B) Turn the left three way valve 90°, withdraw 5 ml of saline/heparin mixed with blood. (C) Turn the middle three way valve 90°, withdraw EDTA sample and place it directly on ice. (D) Turn the right three way valve 90°, withdraw serum sample and let it clot for at least 30 min. (E) Turn the right and middle three way valve 90° and empty the syringe filled with the saline/heparin mixed with blood. (F) Flush with saline and turn the left three way valve 90° and continue with infusion (return to position (A)).