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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Histochem Cell Biol. 2015 Jan 8;143(6):565–574. doi: 10.1007/s00418-014-1304-0

Figure 1. Fractalkine and CX3CR1 expression in the trophoblast cell line BeWo and THP-1 monocytes.

Figure 1

BeWo cells treated either with (a, c) solvent control DMSO (0.2%, 48h) or (b, d) Forskolin (20μM, 48h), were immunohistochemically stained for fractalkine (a, b) or βhCG (c, d). Cytospins of THP-1 monocytes were stained for CX3CR1 (e). RT-PCR revealed fractalkine expression in Forskolin treated BeWo cells (BeWo +F), while no expression was detected in cells treated with solvent control DMSO (BeWo +D) (f). Forskolin induced differentiation of BeWo cells was confirmed by increased expression of βhCG. CX3CR1 expression was detected in THP-1 monocytes, but not in BeWo cells. The expression of ribosomal protein L30 (RPL30) served as endogenous reference. No template control (NTC) did not show any amplification products.