Figure 5.
Immune responses to the rAAV serotypes and to GFP. (a) Quantification of Nab assay reported as the reciprocal of the most dilute serum concentration that blocked infections (+s.e.m.). Note that the antibody response to rAAV2/2 was significantly greater than that to rAAV2/9, there were no other significant differences. (b) Indirect ELISA detecting anti-GFP antibodies in the serum, reported relative to a naïve cat (+s.e.m.). Cats injected with rAAV2/2 and 2/9 showed a significantly higher response to GFP than those injected with rAAV2/5 and 2/8. Both assays were performed in triplicate. For animals exposed to rAAV2/2 and rAAV2/9, n=3 and for AAV2/5 and rAAV2/8, n=2.!
