Table 3.
Antibodies used for immunohistochemistry
| Antibody | Host | Target | Concentration | Source |
|---|---|---|---|---|
| Primary antibodies | ||||
| Cone Arrestin | Rabbit | Cone photoreceptors | 1:10 000 | Dr Cheryl Craft, Doheny Eye Institute, University of Southern California, Los Angeles, CA, USA40 |
| Glutamine Synthetase | Rabbit | Müller cells | 1:1000 | Sigma Aldrich Inc., St Louis, MO, USA |
| Glial Fibrillary Acidic Protein | Rabbit | Activated Müller cells | 1:1000 | DakoCytomation, Carpinteria, CA, USA |
| Protein kinase c-alpha | Mouse | Rod bipolar cells | 1:3000 | BD Biosciences, San Jose, CA, USA |
| Calbindin | Mouse | Horizontal cells | 1:1000 | Swant Immunochemicals, Bellinzona, Switzerland |
| Calretinin | Rabbit | Horizontal cells | 1:1000 | Swant Immunochemicals |
| GFP | Rabbit | Green fluorescent protein | 1:1000 | Invitrogen, Carlsbad, CA, USA |
| Secondary antibodies | ||||
| Alexa Fluor 546 F(ab’)2 fragments of goat anti-rabbit IgG (H+L) | Goat | Rabbit primary antibody | 1:250 | Invitrogen |
| Alexa Fluor594 rabbit anti-mouse IgG (H+L) | Rabbit | Mouse primary antibody | 1:250 | Invitrogen |