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. 2015 Jun 16;13:39. doi: 10.1186/s12915-015-0151-3

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© Ren et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Lhx8 inactivation in primary follicles (Lhx8 ^flx/flx Zp3Cre) abolishes follicle growth. Histomorphological analysis was done on control (Lhx8 ^flx/flx) and Lhx8 deficient ovaries (Lhx8 ^flx/flx Zp3Cre) at various stages of postnatal ovarian development ranging from newborn (PD0) to postnatal day 30 (PD30). a–f Periodic acid–Schiff (PAS) staining and counting of different follicle types in the newborn and PD7 ovaries showed no significant differences between control and Lhx8 deficient ovaries. g–o Anti-NOBOX antibodies were used to stain oocytes (brown immunoreactivity) in ovaries from PD14 (G and H), PD21 (J and K), and PD30 (M and N) mice. At PD14, the Lhx8 ^flx/flx Zp3Cre ovaries showed a significantly higher number of primary follicles (PrF) and significantly diminished number of secondary/preantral (SF/AF) follicles characterized by two or more layers of granulosa cells. At PD21 and PD30, the primary follicle pool did not differ significantly between Lhx8 ^flx/flx Zp3Cre and control ovaries; however, there was a marked decrease in the number of secondary and more advanced ovarian follicles in conditional knockouts including degenerating follicles without oocytes (marked by asterisks in insets in K and N). The primordial follicle (PF) pool remained relatively stable between PD14 and PD30, with no significant difference between Lhx8 ^flx/flx Zp3Cre and control ovaries. **P < 0.01. Scale bars: 100 μm (A, B, D, and E); 200 μm (G, H, J, K, M, and N); 50 μm (insets of K and N)