Table 2.
MRM parameters of the precursor ion, product ion, cone voltage, collision energy and type of fragment for each candidate signature peptide and ISs.
| Peptide | Precursor ion (m/z) | Cone voltage (V) | Product ion (m/z) | Collision energy (eV) | Fragment |
|---|---|---|---|---|---|
| EMPFPK | 374.9 | 15 | 146.1 | 25 | y1 |
| 243.2 | 15 | y2 | |||
| 487.3 | 10 | y4 | |||
| VLPVPQK | 391.0 | 15 | 213.5 | 12 | b2 |
| 371.8 | 18 | y3 | |||
| 568.3 | 12 | y5 | |||
| AVPYPQR | 416.9 | 15 | 175.1 | 26 | y1 |
| 330.7 | 10 | y5 | |||
| 400.2 | 18 | y3 | |||
| DMPIQAFLLYQEPVLGPVR | 730.8 | 20 | 428.0 | 29 | y4 |
| 737.0 | 29 | y7 | |||
| 867.0 | 26 | y8 | |||
| GPFPIIV | 742.9 | 40 | 441.3 | 27 | y4 |
| 512.3 | 27 | b5 | |||
| 625.4 | 23 | b6 | |||
| VL*PV*PQK (IS1) | 397.5 | 15 | 220.3 | 12 | b2 |
| 371.8 | 18 | y3 | |||
| 574.3 | 12 | y5 | |||
| VMPVLK (IS3) | 344.0 | 15 | 231.2 | 10 | b2 |
| 260.2 | 17 | y2 | |||
| 456.3 | 12 | y4 |