Frequency of infection with “Candidatus Rickettsia andeanae” among 216 questing adult Amblyomma maculatum ticks collected from Kansas (Anderson, Butler, Crawford, Geary, Morris, Neosho, Osage, Riley, and Shawnee counties) and Oklahoma (Cleveland, Cotton, Kiowa, Lincoln, Osage, Payne, Tillman, Tulsa, and Washington counties) during 2011–2014. Fractions represent the numbers of ticks infected with “Ca. R. andeanae” over the number of ticks that were evaluated by the real-time PCR assay. No molecular evidence of infection with Rickettsia parkeri was identified in any specimen of A. maculatum in either state.