Extended Data Figure 5. MUC1-mediated adhesion formation.

a, Quantification of the average number of large adhesions, greater than 1 µm², per area of cell in control epithelial cells (Control) and those ectopically expressing ectodomain-truncated MUC1 (+MUC1(ΔTR)), wild-type MUC1 (+MUC1), or cytoplasmic-tail-deleted MUC1 (+MUC1(ΔCT)). Results are the mean ± s.e.m. of three separate experiments. b, Fluorescence micrographs showing immuno-labelled MUC1 and fluorescently labelled fibronectin fibrils in control and MUC1-expressing epithelial cells. Soluble, labelled fibronectin in the growth media was deposited by cells at sites of cell–matrix adhesion. Binding of soluble fibronectin to MUC1 was not detected. Scale bar, 10 µm. c, Time lapse images of MUC1–YFP and vinculin–mCherry, showing the dynamics of adhesion assembly (Vinc.) and MUC1 patterning (MUC1). Scale bar, 1 µm. d, Rate of adhesion measured with single cell force spectroscopy of control (Cont.), α₅ integrin-blocked (anti-α₅), and MUC1-expressing cells (+MUC1) to fibronectin-coated surfaces and control cells to BSA-coated surfaces (BSA). Results are the mean ± s.e.m. of at least 15 cell measurements. Statistical significance is given by *P < 0.05; **P < 0.01; ***P < 0.001.