Table 2.
Spectral counting and protein ratios for differentially expressed proteins
| Protein | Gene | NSAFa | NSAFa | Rsc b |
|---|---|---|---|---|
| NSCLC | Control | |||
| carbonic anhydrase 2 | CA2 | 0 | 0,00693 | 3,10 |
| MHC class I antigen | HLA-DQB | 0 | 0,0131 | 2,43 |
| alcohol dehydrogenase beta subunit | ADH1B | 0 | 0,00356 | 2,43 |
| carbonic anhydrase 1 | CA1 | 0,00559 | 0,0180 | 2,10 |
| profilin-1 | PFN1 | 0 | 0,00513 | 1,94 |
| MHC class II antigen | HLA-DRB1 | 0 | 0,00931 | 1,94 |
| rho GDP-dissociation inhibitor | ARHGDIB | 0 | 0,00393 | 1,94 |
| aldolase A | ALDOA | 0,00591 | 0,000974 | -1,61 |
| ribosomal protein L7 | RPL7 | 0,00869 | 0,00143 | -1,61 |
| enoyl-CoA hydratase | EHHADH | 0,00250 | 0 | -1,97 |
| lactoferrin precursor | LTF | 0,0124 | 0,00154 | -2,25 |
| proteasome subunit alpha type-6 | PSMA6 | 0,0174 | 0,00144 | -2,52 |
| Aldo-keto reductase family 1 member C4 | AKR1C4 | 0,00579 | 0 | -2,71 |
| stomatin | STOM | 0,00604 | 0 | -2,71 |
| 60S acidic ribosomal protein P0 | RPLP0 | 0,00807 | 0 | -3,20 |
| phosphoglycerate mutase 1 | PGAM1 | 0,0107 | 0 | -3,20 |
| endoplasmic reticulum protein 29, isoform CRA_b | ERP29 | 0,00894 | 0 | -3,20 |
| myeloperoxidase, isoform CRA_d | MPO | 0,0184 | 0,00076 | -3,48 |
| L-lactate dehydrogenase A isoform 1 | LDHA | 0,00859 | 0 | -3,57 |
| azurocidin | AZU1 | 0,0161 | 0 | -3,86 |
aNormalized Spectral Abundance Factor
bRsc is calculated according to semi-quantitative parameter proposed by Old [42] and represents the log2 ratio between the protein expression level of control vs the protein expression level of NSCLC tissues. Proteins with Rsc ≥ 1,50 or ≤ -1,50 were considered differentially expressed