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(a) VANGL1 expression of HEP-G2 cells transfected with pSilencer plasmid. pSIL-VANGL1 cells carry the shRNA template for VANGL1 and pSIL where the control cells transfected with the control pSilencer vector. Relative quantification of VANGL1 expression with real-time PCR in transfected cells showing the degree of gene silencing (p=0.0009). *Indicates the significance. (b) Western blot analysis of VANGL1 expression in the cells transfected with siRNA.
