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Identification of PTMs on HEXIM1 by tandem mass spectrometry. (A) Cartoon based on the published NMR structure of the coiled-coil dimeric region of HEXIM1 [26] showing the locations of the phospho-sites S268, T270, Y271, Y274, and S355 relative to the coiled-coil dimer. (B) Combined MS/MS sequence coverage of trypsin (in red) and pepsin A (in blue) cleaved HEXIM1 affinity purified from Jurkat T cells.
