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Effect of mutations in HEXIM1 on HIV transcription. (A) Jurkat 2D10 cells carrying stably expressed HEXIM1 were cultured in 20% FBS complete medium and activated as shown for 18 h and then analyzed by flow cytometry for proviral EGFP reporter gene expression due toHEXIM WT, YYEE, or YYFF. (B) Effect of mutating Ser158 on HIV reactivation. Cells were cultured in 5% FBS and treated as indicated. Error bars denote +/− standard error of the mean from at least 3 independent replicates.
