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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Proteomics. 2015 May 15;15(12):2078–2086. doi: 10.1002/pmic.201500038

Figure 5. Effect of Y271E/Y274E on the subcellular localization of HEXIM1 and P-TEFb.

Figure 5

(A) The Y271E/Y274E mutation does not adversely interfere with the nuclear localization pattern of HEXIM1 in Jurkat T cells. Uninfected Jurkat T cells or cells stably expressing FLAG HEXIM1 WT or the YYEE mutant from the integrated MSCV were fixed in 4% formaldehyde, permeabilized and then immunostained with fluorochrome-conjugated antibodies to HEXIM1 or the FLAG epitope. Images were obtained using a DeltaVision deconvolution microscope at 60X magnification. Scale bar represents a length of 30 μM. (B) Immunofluorescence microscopy to examine effect of the YYEE mutation on the subcellular localization of the CDK9 subunit of P-TEFB. Uninfected Jurkat T cells or cells stably expressing FLAG HEXIM1 WT or the YYEE mutant from the integrated MSCV were fixed in 4% formaldehyde, permeabilized and then immunofluorescently stained for CDK9. Images were obtained using a DeltaVision deconvolution microscope at 60X magnification. Scale bar represents a length of 15 μM.