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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Bone Miner Res. 2014 Oct;29(10):2262–2275. doi: 10.1002/jbmr.2256

Figure 6. The TH effect on chondro/osteoblast differentiation is dependent on Osx expression.

Figure 6

[A] T3 treatment increases ALP staining in ATDC5 cells. [B] TH stimulates expression of osteoblast markers in ATDC5 chondrogenic lineage cells. [C] T3 stimulates formation of mineralized nodules in ATDC5 cells. [D–G] Knockdown of Osx expression impairs chondrogenic cell differentiation into chondro/osteoblasts. ATDC5 cells were infected with lenti-virus expressing shRNA against Osx or GFP. The cells were then cultured in a serum-free medium with or without T3 for 24 hours prior to harvesting RNA for extraction and real time RT-PCR. Parallel cultures were also lysed for measurement of ALP activity. Values are expressed as fold change of vehicle treated ATDC5 cells transfected with GFP shRNA produced from lentivirus. An asterisk indicates a significant difference in T3 compared to vehicle treatment (P < 0.01). “a” indicates a significant difference in T3 treated ATDC5 cells transfected with GFP shRNA (P<0.01).). “b” indicates a significant difference compared to 0.1 ng/ml T3 treatment (P<0.01). “c” indicates a significant difference compared to 1 ng/ml T3 treatment (P<0.01).