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. Author manuscript; available in PMC: 2016 Jun 18.
Published in final edited form as: Chem Biol. 2015 Jun 18;22(6):776–784. doi: 10.1016/j.chembiol.2015.05.008

Figure 3.

Figure 3

TGF-α induces an ‘inside-out’ helical interaction in the JM-A.

(A) Helical wheel diagrams illustrating two anti-parallel interfaces potentially adopted in the presence of TGF-α.

(B) JM-A regions of CCH-9 and CCH-10.

(C,E) TIRF images of CHO-K1 cells expressing either FLAG-tagged CCH-9 or CCH-10 (green fluorescence) and treated with EGF or TGF-α (1 ng/mL) and ReAsH. Bars represent 10 µm.

(D,F) Quantified fold increase in expression-corrected ReAsH fluorescence over background cells expressing CCH-9 or CCH-10 and treated with or without EGF or TGF-α. We attribute the slightly lower ReAsH fluorescence of CCH-10 to the lower activity of this mutant (see also Figure S4E). Error bars represent standard error. **** p < 0.0001 from one way ANOVA with Bonferroni post-test (see also Figure S4).