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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Endocr Relat Cancer. 2014 Sep 16;21(6):865–877. doi: 10.1530/ERC-14-0424

FIGURE 1.

FIGURE 1

PAK function is BRAF-dependent. A. BRAF siRNA blocked PAK-mediated phosphorylation of vimentin and MEK in BCPAP, TPC1 and FTC133 cell lines versus scrambled sequence control. Transfection efficiencies were approximately 40-60%. “Longer” refers to a longer exposure of the same blot for adequate visualization of the bands of all cell lines for the pMEK immunoblot. B. Expression of a molecular inhibitor of group 1 PAKs, GFP-tagged PID, did not reduce BRAF-mediated MEK1/2 phosphorylation in all three-thyroid cancer cell lines versus vector transfection control. Representative Western blots are shown for experiments repeated independently on at least two occasions.