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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Endocr Relat Cancer. 2014 Sep 16;21(6):865–877. doi: 10.1530/ERC-14-0424

FIGURE 3.

FIGURE 3

MEK and group I PAKs regulate thyroid cancer cell motility. A. MEK1/2 and group I PAK activity were inhibited using U0126 (2µM) or DMSO and GFP-PID or GFP-control, respectively. Migration experiments showed that inhibiting both MEK and group I PAKs independently and simultaneously significantly inhibited migration to a similar degree (p-values as shown in figure). B. Western Blot confirmed inhibition of MEK activity by U0126 and successful transfection of the PID and control vector by immunoblot for phosphorylated ERK and GFP, respectively. The transfection efficiency was approximately 30-40% based on imaging of GFP.